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M94A1884.TXT
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1994-10-24
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Document 1884
DOCN M94A1884
TI Molecular mimicry between V3 loop of HIV-1 gp120 and endogenous human
proteins.
DT 9412
AU Trujillo JR; McLane MF; Lee TH; Essex M; Harvard School of Public
Health, Cancer Bio. Dept., Boston, MA.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):43 (abstract no. 144A). Unique
Identifier : AIDSLINE ICA10/94370691
AB OBJECTIVE: Viral infections can induce autoimmune responses by several
mechanisms including molecular mimicry. To determine the role of the
cellular immune response in HIV pathogenesis, antibodies to gp120, a
highly immunogenic protein of HIV-1, were analyzed for cross-reactivity
with endogenous human proteins. METHODS AND RESULTS: Homogenized brain,
kidney, liver, lung and spleen tissues from HIV-1 seronegative
individuals as well as lysates from various cell lines were fractionated
by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Initial western blot analyses of human brain proteins (HBP) with several
monoclonal and polyclonal anti-HIV-1 antibodies including anti-HIV-1
gp120, anti-gp120 V3 loop (V3mAb), anti-gp120 V5 loop, anti-gp41,
anti-p24, anti-p17, anti-Vpu and anti-Vpr revealed cross-reactivity only
with the V3mAb. The V3mAb specifically reacted with three prominent
human brain proteins of approximately 35, 55 and 110 kDa and with minor
proteins of 23 and 65 kDa. The 55 kDa human brain protein appears to be
specific to the central nervous system. Western blot analyses of
proteins from peripheral tissues and various cell lines were performed
using the V3mAb and revealed reactivity to 35 and 110 kDa proteins.
Binding of the V3mAb to endogenous human proteins appears to be specific
since it was competitively blocked by recombinant HIV-1 gp120.
DISCUSSION AND CONCLUSIONS: The V3 loop of HIV-1 gp120 appears to share
an epitope with endogenous human proteins. An immune response to the V3
loop that can generate cross-reactive antibodies to cellular proteins
may be an autoimmune mechanism by which HIV-1 can damage cells within
the central nervous system and other organs.
DE Antibodies, Monoclonal Antigenic Determinants/IMMUNOLOGY/ISOLATION &
PURIF Autoantibodies/BIOSYNTHESIS Autoantigens/ISOLATION & PURIF
Binding, Competitive Brain/IMMUNOLOGY Cell Line Cross Reactions
Human HIV Antibodies HIV Antigens HIV Envelope Protein
gp120/*IMMUNOLOGY HIV Infections/IMMUNOLOGY HIV-1/*IMMUNOLOGY
Immunity, Cellular Molecular Weight Nerve Tissue
Proteins/CHEMISTRY/IMMUNOLOGY Peptide Fragments/*IMMUNOLOGY
Proteins/*IMMUNOLOGY/ISOLATION & PURIF MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).